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. 2020 Jan 8;45(3):703–714. doi: 10.3892/ijmm.2020.4459

Figure 4.

Figure 4

Silencing of PLAG1 and IGF2 in A2780 using siRNA. (A) Gene expression of PLAG1 and IGF2 in A2780, SKOV3, HO8910 and COC2 cell lines; *P<0.05 vs. A2780. PLAG1 (B) mRNA expression, (C) western blot analysis and (D) protein quantification for OC cells transfected with three si-PLAG1s *P<0.05 vs. NC-1; #P<0.05 vs. si-PLAG1-3. IGF2 (E) mRNA expression, (F) western blot analysis and (G) protein quantification for OC cells transfected with three si-PIGF2s *P<0.05 vs. NC-2; #P<0.05 vs. si-PIGF2-3. Data are presented as the mean ± standard error; each experiment was repeated three times. OC, ovarian cancer; PLAG1, pleomorphic adenoma gene 1; IGF2, insulin-like growth factor-2; si, small interfering RNA; NC, negative control.