Table 1.
Reagents and resources.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Vinculin | Sigma-Aldrich | Cat#V4505 |
| SLC7A11/xCT (D2M7A) | Cell Signaling Technology | Cat#12619 |
| phospho-p53 (Ser15) | Cell Signaling Technology | Cat#9284 |
| ACSL4 | Santa Cruz | Cat#sc-271800 |
| KEAP1 | Santa Cruz | Cat#sc-365626 |
| GPX4 | R&D Systems | Cat#MAB5457 |
| Tubulin | Cell Signaling Technology | Cat#2144 |
| phospho-Chk2 (Thr68) | Cell Signaling Technology | Cat#2197 |
| phospho-histone H2A.X (Ser139) | EMD Millipore | Cat#05-636 |
| Anti-4 Hydroxynonenal (4-HNE) | Abcam | Cat#ab46545 |
| Ki-67 (D2H10) | Cell Signaling Technology | 9027S |
| Cleaved Caspase-3 (Asp175) | Cell Signaling Technology | 9661s |
| Chemicals | ||
| Erastin | Sigma | Cat#E7781 |
| Ferrostatin-1 | Sigma | Cat#SML0583 |
| Liproxstatin-1 | Sigma | Cat#SML1414 |
| N-acetyl-L-cysteine | Sigma | Cat#A9165 |
| Sulfasalazine | Sigma | Cat#S0883 |
| Z-VAD-fmk | R&D Systems | Cat#FMK001 |
| Necrostatin-1s | BioVision | Cat#2263 |
| RSL3 | Selleckchem | Cat#S8155 |
| FIN56 | Cayman Chemical | Cat#25180 |
| ML162 | Cayman Chemical | Cat#20455 |
| CM-H2DCFDA | ThermoFisher | Cat#C6827 |
| BODIPY 581/591 C11 | Invitrogen | Cat#D3861 |
| Critical Commercial Assays | ||
| Cell Counting Kit-8 (CCK8) reagent | Dojindo Molecular Technologies | Cat#CK04 |
| Experimental Models: Cell Lines | ||
| A549 | ATCC | Cat#CCL-185 |
| H460 | ATCC | Cat#HTB-177 |
| H1299 | ATCC | Cat#CRL-5803 |
| H23 | ATCC | Cat#CRL-5800 |
| MCF7 | ATCC | Cat#HTB-22 |
| HT-1080 | ATCC | Cat#CCL-121 |
| FLO-1 | Dr. Steven H. Lin at MD Anderson Cancer Center | |
| UMRC6 | Dr. William G. Kaelin at Dana-Farber Cancer Institute | |
| HEK293T | ATCC | Cat#CRL-11268 |
| Experimental Models: Organisms/Strains | ||
| Mouse: athymic nude: Foxn1nu/nu | The facility in the Department of Experimental Radiation Oncology at MD Anderson Cancer Center | |
| NOD scid gamma (NSG) mice | The facility in the Department of Experimental Radiation Oncology at MD Anderson Cancer Center | |
| Sequence-Based Reagents | ||
| For primer and guide RNA sequences, please see Supplementary information, Table S2. | ||
| Software and Algorithms | ||
| GraphPad Prism 6 | GraphPad Software, Inc. | |
| FlowJo_V10 | FlowJo, LLC | |
| CompuSyn | CompuSyn, Inc. | |
| ImageJ | NIH | |
| SPSS 25.0 | SPSS Inc. | |