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. 2020 Jan 20;45(3):858–872. doi: 10.3892/ijmm.2020.4471

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Copyright: © Han et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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LncRNA GAS5 alleviates the apoptosis caused by hypoxia/reoxygenation. (A) Evaluation of apoptotic cells by Hoechst staining. Left panel: The nucleus of apoptotic cells is densely stained; the arrow points to apoptotic cells. Right panel: Quantified apoptosis rate. (B) Left panel: Evaluation of apoptotic cells by flow cytometry. Right panel: Quantified early apoptosis rate and late apoptosis rates. (C) Protein expression of Bcl2 in H9c2 cells detected by western blotting. (D) Protein expression of Bax in H9c2 cells detected by western blotting. (E) Quantitative value of relative protein expression levels of Bcl-2. (F) Quantitative value of relative protein expression levels of Bax. (G) Ratio of protein expression of Bcl-2/Bax in H9c2 cells. (H) Protein expression of cleaved caspase 3 in H9c2 cells detected by western blotting. (I) Quantitative value of relative protein expression levels of cleaved caspase 3. ^*P<0.05, ^**P<0.01 and ^***P<0.001 with comparisons shown by lines. Data are presented as the mean ± standard deviation; n=3. LncRNA, long non-coding RNA; GAS5, growth arrest specific 5; si/siRNA, small interfering RNA; NC, negative control; HR, hypoxia-reoxygenation; Bax, Bcl-2-associated X protein; Bcl2, B-cell lymphoma 2.

LncRNA GAS5 alleviates the apoptosis caused by hypoxia/reoxygenation. (A) Evaluation of apoptotic cells by Hoechst staining. Left panel: The nucleus of apoptotic cells is densely stained; the arrow points to apoptotic cells. Right panel: Quantified apoptosis rate. (B) Left panel: Evaluation of apoptotic cells by flow cytometry. Right panel: Quantified early apoptosis rate and late apoptosis rates. (C) Protein expression of Bcl2 in H9c2 cells detected by western blotting. (D) Protein expression of Bax in H9c2 cells detected by western blotting. (E) Quantitative value of relative protein expression levels of Bcl-2. (F) Quantitative value of relative protein expression levels of Bax. (G) Ratio of protein expression of Bcl-2/Bax in H9c2 cells. (H) Protein expression of cleaved caspase 3 in H9c2 cells detected by western blotting. (I) Quantitative value of relative protein expression levels of cleaved caspase 3. ^*P<0.05, ^**P<0.01 and ^***P<0.001 with comparisons shown by lines. Data are presented as the mean ± standard deviation; n=3. LncRNA, long non-coding RNA; GAS5, growth arrest specific 5; si/siRNA, small interfering RNA; NC, negative control; HR, hypoxia-reoxygenation; Bax, Bcl-2-associated X protein; Bcl2, B-cell lymphoma 2.