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. 2020 Jan 20;45(3):858–872. doi: 10.3892/ijmm.2020.4471

Figure 4.

Figure 4

LncRNA GAS5 functions as a molecular sponge for miR-532-5p in H9c2 cells. (A) Prediction of miR-532-5p binding sites on the lncRNA GAS5 transcript and the Luciferase assay mutated sequence. (B) Expression levels of miR-532-5p were detected under HR treatment and the simultaneous inhibition of lncRNA GAS5 by PCR. (C) Transfection efficiency of miR-532-5p mimic. ***P<0.001 vs. the control. (D) Transfection efficiency of miR-532-5p inhibitor. ***P<0.001 vs. the control. (E) Expression levels of lncRNA GAS5 were detected following the intervention of miR-532-5p with HR treatment by PCR. (F) Combination between lncRNA GAS5 and miR-532-5p was demonstrated using a luciferase reporter assay. **P<0.01 and ***P<0.001 with comparisons shown by lines. Data are presented as the mean ± standard deviation. n=3. LncRNA, long non-coding RNA; GAS5, growth arrest specific 5; miR, microRNA; HR, hypoxia-reoxygenation; NC, negative control; WT, wild type; MUT, mutant.