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. 2020 Jan 20;45(3):858–872. doi: 10.3892/ijmm.2020.4471

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Copyright: © Han et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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LncRNA GAS5 regulates PTEN through miR-532-5p in HR injury. (A) Gene expression of PTEN following lncRNA GAS5 intervention. (B) Gene expression of PTEN following miR-532-5p intervention. (C) Protein expression of PTEN following lncRNA GAS5 intervention. (D) Quantitative value of relative protein expression levels of PTEN. (E) Protein expression of PTEN following miR-532-5p intervention. (F) Quantitative value of relative protein expression levels of PTEN. (G) Prediction of miR-532-5p binding sites on the PTEN transcript. Luciferase assay mutated sequence. (H) Association between PTEN and miR-532-5p was demonstrated using a luciferase reporter assay. ^*P<0.05, ^**P<0.01 and ^***P<0.001 with comparisons shown by lines. Data are presented as the mean ± standard deviation. n=3. LncRNA, long non-coding RNA; GAS5, growth arrest specific 5; PTEN, phosphatase and tensin homolog; miR, microRNA; HR, hypoxia-reoxygenation; si, small interfering RNA; NC, negative control; WT, wild type; MUT, mutant; 3′UTR, 3′ untranslated region.

LncRNA GAS5 regulates PTEN through miR-532-5p in HR injury. (A) Gene expression of PTEN following lncRNA GAS5 intervention. (B) Gene expression of PTEN following miR-532-5p intervention. (C) Protein expression of PTEN following lncRNA GAS5 intervention. (D) Quantitative value of relative protein expression levels of PTEN. (E) Protein expression of PTEN following miR-532-5p intervention. (F) Quantitative value of relative protein expression levels of PTEN. (G) Prediction of miR-532-5p binding sites on the PTEN transcript. Luciferase assay mutated sequence. (H) Association between PTEN and miR-532-5p was demonstrated using a luciferase reporter assay. ^*P<0.05, ^**P<0.01 and ^***P<0.001 with comparisons shown by lines. Data are presented as the mean ± standard deviation. n=3. LncRNA, long non-coding RNA; GAS5, growth arrest specific 5; PTEN, phosphatase and tensin homolog; miR, microRNA; HR, hypoxia-reoxygenation; si, small interfering RNA; NC, negative control; WT, wild type; MUT, mutant; 3′UTR, 3′ untranslated region.