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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Mol Oral Microbiol. 2019 Dec 26;35(1):29–39. doi: 10.1111/omi.12275

Figure 4. Effect of EGCg on LtxA Association with AA1704 OMVs.

Figure 4.

AA1704 OMVs were incubated with purified LtxA, untreated or pretreated with EGCg, for 15 mins at room temperature. OMV-associated and unbound LtxA were separated by SEC. One-mL fractions were collected, and the lipid concentration (white circles, primary y-axis) in each fraction was quantified using the FM 4–64 dye, and the LtxA concentration (black squares, secondary y-axis) was quantified using an immunoblot. (A) AA1704 OMVs incubated with untreated LtxA. (B) AA1704 OMVs incubated with EGCg-treated LtxA.