Figure 2.

Effects of miR-20b expression level on cell senescence. (A) The cells were transfected with the miR-20b mimic, miR-20b inhibitor or scramble and the transfection rate was detected by reverse transcription-quantitative PCR. (B) The effect of miR-20b on cell viability was detected by Cell Counting Kit-8. (C) The effect of miR-20b on cell senescence was detected by SA-β-gal staining. The HUVECs were divided into 6 groups, namely the scramble group, miR-20b mimic group, miR-20b inhibitor group, scramble + H₂O₂ group, miR-20b mimic + H₂O₂ group and miR-20b inhibitor + H₂O₂ group. The cells were transfected with the miR-20b mimic, miR-20b inhibitor or scramble and then stimulated with H₂O₂ (100 µM), or left untreated. ^**P vs. scramble; ##P<0.001 vs. scramble + H₂O₂. HUVECs, human umbilical vein endothelial cells; miR, microRNA; SA-β-gal, SA-β-galactosidase; OD, optical density.