Figure 3.

Target gene of miR-20b was predicted. (A) The potential target genes of miR-20b were predicted by Targetscan (http://www.targetscan.org/) and RT-qPCR was performed for detection. ^**P<0.001 vs. scramble; ^##P<0.001 vs. scramble + H₂O₂. (B) The binding sites of the target gene and miR-20b were predicted by Targetscan. (C) The binding abilities of miR-20b and TXNIP were detected by dual luciferase reporter. ^**P<0.001 vs. Control + TXNIP-3′-UTR; ^##P<0.001 vs. miR-20b mimic + TXNIP-3′-UTR mut. (D) The binding abilities of miR-20b and NLRP3 were detected by dual luciferase reporter. ^**P<0.001 vs. Control + TXNIP-3′-UTR; ^##P<0.001 vs. miR-20b mimic + TXNIP-3′-UTR mut. HUVECs were divided into 8 groups, namely, Control + TXNIP-3′-UTR group, miR-20b mimic + TXNIP-3′-UTR group, Control + TXNIP-3′-UTR mut group, miR-20b mimic + TXNIP-3′-UTR mut group, Control + NLRP3-3′-UTRgroup, miR-20b mimic + NLRP3-3′-UTR group, Control + NLRP3-3′-UTR mut group and miR-20b mimic + NLRP3-3′-UTR mut group. The pGL3 control vectors were used to generate recombinant vectors with mut sequence or a 3′UTR sequence of TXNIP or NLRP3 and the recombinant vectors and/or miR-20b mimic were transfected into cells. Mut, mutant; UTR, untranslated region; NLRP3, NLR family pyrin domain containing 3; HUVECs, human umbilical vascular cell; TXNIP, thioredoxin interacting protein.