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. 2020 Jan 23;45(3):886–896. doi: 10.3892/ijmm.2020.4474

Figure 5.

Figure 5

WNT2B is a key target of miR-137 in cholangiocarcinoma. (A) Bubble chart showing the pathways of the miR-137 target genes were enriched in. (B) miR-137 may bind to the 3'-UTR of WNT2B mRNA. The underlined sequence is the mutated site. (C) miR-137 mimics inhibited luciferase activity in cholangiocarcinoma cells, while mutation of the 3'-UTR of WNT2B mRNA abolished the effect of miR-137 mimic on luciferase activity. (D) Overexpression of miR-137 decreased the mRNA expression level of WNT2B in cholangiocarcinoma cells. (E) The expression of miR-137 was inversely associated with that of WNT2B in cholangiocarcinoma tissues. (F) The mRNA expression levels of WNT2B were detected in 29 cholangiocarcinoma tissues and 20 adjacent tissues using reverse transcription-quantitative PCR. (G) The mRNA expression levels of WNT2B in the cholangiocarcinoma cell lines TFK-1, HuCCT1, RBE and QBC939 and HIBEpiCs were detected using reverse transcription-quantitative PCR. **P<0.01. WNT2B, Wnt family member 2B; UTR, untranslated region; HIBEpiCs, human intrahepatic biliary epithelial cells; NC, negative control; LV, lentivirus; miR, microRNA.