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. 2020 Jan 9;45(3):769–778. doi: 10.3892/ijmm.2020.4460

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Copyright: © Shu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Exposure to HMGB1 promotes chondrocyte apoptosis. (A) Hoechst 33342 staining was performed on ATDC5 cells treated with HMGB1 (0, 10, 50, 100 and 300 ng/ml) or IL-1β (positive control group). Apoptotic cells exhibited morphological changes in the nuclei typical of apoptosis. Images were captured under a fluorescence microscope. Arrows, apoptotic ATDC5 cells. Scale bar, 20 µm. The quantified data are shown in (B) ^*P<0.05 compared with the 0 concentration group. (C) Cells were treated as in (A) and flow cytometry assay was performed; the quantified data are shown in (D). ^*P<0.05 compared with the 0 concentration group. (E and F) Cells were treated with HMGB1 (100 ng/ml) or IL-1β (5 ng/ml). Alcian blue staining was performed on ATDC5 cells and the stained area was quantified. ^*P<0.05 compared with control. HMGB1, high mobility group box 1 protein; IL-1β, interleukin 1β.

Exposure to HMGB1 promotes chondrocyte apoptosis. (A) Hoechst 33342 staining was performed on ATDC5 cells treated with HMGB1 (0, 10, 50, 100 and 300 ng/ml) or IL-1β (positive control group). Apoptotic cells exhibited morphological changes in the nuclei typical of apoptosis. Images were captured under a fluorescence microscope. Arrows, apoptotic ATDC5 cells. Scale bar, 20 µm. The quantified data are shown in (B) ^*P<0.05 compared with the 0 concentration group. (C) Cells were treated as in (A) and flow cytometry assay was performed; the quantified data are shown in (D). ^*P<0.05 compared with the 0 concentration group. (E and F) Cells were treated with HMGB1 (100 ng/ml) or IL-1β (5 ng/ml). Alcian blue staining was performed on ATDC5 cells and the stained area was quantified. ^*P<0.05 compared with control. HMGB1, high mobility group box 1 protein; IL-1β, interleukin 1β.