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. 2020 Jan 10;45(3):873–885. doi: 10.3892/ijmm.2020.4463

Figure 6.

Figure 6

PQQ alters the levels of ROS in AC16 cells. (A) Effect of PQQ on Iso-induced ROS levels in AC16 cells was detected with the Reactive Oxygen Species Assay kit and then photographed (groups were set as described in the 'Materials and methods'). (B) The levels of ROS were quantified by ImageJ software, with the mean fluorescence intensity of the control group set as 1. (C) DCFH-DA fluorescent dye was used to detect ROS production in the AC16 cells with a flow cytometer. PQQ attenuated Iso-induced ROS levels in AC16 cells. Black line, control group; red line, Iso group; gray line, 1 µM PQQ + Iso group; blue line, 2.5 µM PQQ + Iso group; yellow line, 5 µM PQQ + Iso group. (D) Statistical data are presented based on experiments performed in triplicate. Data are presented as the means ± SD (n=3). #P<0.05 vs. the control group, *P<0.05 vs. the Iso group. PQQ, pyrroloquinoline quinone; Iso, isoproterenol hydrochloride; ROS, reactive oxygen species.