Table I.

Reaction system of first-strand cDNA synthesis.

Reagent	Volume (µl)
5X PrimeScript Buffer (for real-time)	2
PrimeScriptRT Enzyme Mix I	0.5
Oligo dT Primer (50 µM)	0.5
Random 6 mers (100 µM)	0.5
Total RNA	x
RNase Free dH2O	6.5-x
Total volume per rxn	10