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Stacked paper based 3D culture platform. Cells are spotted on paper (2–8 mm radius spots). The layers can then either be stacked to mimic a nutrient system (limited by diffusion) or left free to mimic a diffusion-unlimited 3D cell culture system. The stacked system can be disassembled for analysis. (Adapted from Ref 66 with permission from the Proceedings of the National Academy of Science)
