KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat monoclonal anti-mouse/human GL7 antigen-PacBlue (clone GL7) | Biolegend | Cat# 144613; RRID: AB_2563291 |
| Rat monoclonal anti-mouse/human CD45R/B220-PerCP/Cy5.5 | Biolegend | Cat# 103235; RRID: AB_893356 |
| Mouse monoclonal anti-mouse CD45.1-FITC (clone A20) | Biolegend | Cat# 110705; RRID: AB_313494 |
| Mouse monoclonal anti-mouse CD45.2-APC (clone 104) | Biolegend | Cat# 109813; RRID: AB_389210 |
| Rat monoclonal anti-mouse CD16/32 PE | Biolegend | Cat# 101307; RRID: AB_312806 |
| Rat monoclonal anti-mouse CD16/32 Alexafluor 647 | Biolegend | Cat# 101314; RRID: AB_2278396 |
| Rat monoclonal anti-mouse CD35 biotin | BD Biosciences | Cat# 553816; RRID: AB_395068 |
| Rat monoclonal anti-mouse Ki-67 FITC | Biolegend | Cat# 652409; RRID: AB_2562140 |
| Streptavidin- Pacific Blue | ThermoFisher Scientific | Cat# S11222 |
| Rat monoclonal anti-mouse CD169 PE | Biolegend | Cat# 3142403; RRID: AB_10915470 |
| Rat monoclonal anti-mouse CD38 PE/Cy7 | Biolegend | Cat# 102717; RRID: AB_2072892 |
| Rat monoclonal anti-mouse CD54 (ICAM) PE | Biolegend | Cat# 116107; RRID: AB_313698 |
| Rat monoclonal anti-mouse CD106(VCAM) FITC | Biolegend | Cat# 105705; RRID: AB_313206 |
| Rat monoclonal anti-mouse CD138 PE | Biolegend | Cat# 142503; RRID: AB_10915989 |
| Mouse monoclonal anti-idiotype (clone 9D11) | (Chatterjee et al., 2013) | N/A |
| Rat monoclonal anti-mouse IgD biotin | Biolegend | Cat# 405733; RRID: AB_2563343 |
| Armenian Hamster monoclonal anti-mouse CD3e biotin | Biolegend | Cat# 100304; RRID: AB_312669 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| B - Phycoerythrin | Anaspec | Cat# AS-82001 |
| Tamoxifen | SigmaAldrich | Cat# T5648–5G |
| Sunflower Seed oil | Spectrum Chemical | Cat# S1929–500MLGL |
| Fixable Viability Dye eFluor® 780 | Thermofisher Scientific | Cat# 65–0865-18 |
| Imject Alum Adjuvant | Thermofisher Scientific | Cat# 77161 |
| Np-Osu | Bioresearch technologies | Cat# N-1010–100 |
| Chicken gamma globulin | Rockland Immunochemicals | Cat# D602–0100 |
| Experimental Models: Cell Lines | ||
| T. ni: High Five | ThermoFisher | Cat# B85502 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Aid-CreERT2 EYFP: AicdaCreERT2 flox-stop-flox-EYFP | (Dogan et al., 2009) | N/A |
| Mouse: FcgRIIB flox/flox | (Boross et al., 2011) | N/A |
| Mouse: PA-GFP: B6.Cg-Ptprca Tg(UBC-PA-GFP)1Mnz/J | The Jackson Laboratory | JAX:022486 |
| Mouse: 564Igi: 564 HiKi | (Berland et al., 2006) | N/A |
| Mouse: Aid-CreERT2 Confetti: AicdaCreERT2-Rosa26Confetti | (Tas et al., 2016) | N/A |
| Mouse: C57BL/6J | The Jackson laboratory | JAX: 000664 |
| Mouse: B6.SJL-Ptprca Pepcb/BoyJ | The Jackson laboratory | JAX: 002014 |
| Oligonucleotides | ||
| Primers for Ig PCR and sequencing | (Busse et al., 2014; Degn et al., 2017; Tiller et al., 2009) | N/A |
| Recombinant DNA | ||
| pFB_H3_Aichi_2_1968_(X31)_FLsE | (Bajic et al., 2019) | N/A |
| Software and Algorithms | ||
| Igblast tools | This paper | https://doi.org/10.5281/zenodo.3483838 |
| FIJI (ImageJ) | (Schindelin et al., 2012) | https://fiji.sc |
| CellProfiler | (Lamprecht et al., 2007) | https://cellprofiler.org/ |
| Python | Python Software Foundation | https://www.python.org |
| Other | ||
| Agencourt RNAClean XP | Beckman Coulter | Cat# A63987 |
| Maxima H Minus Reverse Transcriptase | Thermo Fisher Scientific | Cat# EP0752 |
| TCL buffer | QIAGEN | Cat# 1031576 |