Skip to main content
. Author manuscript; available in PMC: 2020 Apr 14.
Published in final edited form as: Nat Immunol. 2019 Oct 14;20(11):1506–1516. doi: 10.1038/s41590-019-0505-z

Figure 3. Blockade of fluid absorption alters HEV structure in PPs.

Figure 3.

(a) Representative images taken by confocal microscopy of an HEV from the PP of an untreated control mouse or a mouse treated for 3 days with PEG in drinking water. left and middle panels: HEV ECs and FRCs were marked by staining with anti-MAdCAM1 and anti-PDPN respectively. right panel: Contact points between HEV perimeter and surrounding perivascular FRCs. Scale bars = 20um. Bar graph: Total quantification of FRC:HEV contact points from confocal imaging. Data represented as mean ± SEM of three independent experiments (n=10 mice per treatment). Unpaired two tailed Student’s t test was used to compare groups (** P ≤ 0.01). (b) Transmission electron micrograph of PP HEVs. top panel: control. bottom panel: treated for 3 days with PEG in drinking water. ECs false colored in red. Separations between endothelial cell and basement membrane marked by yellow arrows. Lymphocytes marked by “L”. Scale bar = 2um. Representative of two independent experiments. (c) Schematic representation of HEV structure in PPs from untreated (upper) and PEG-treated (lower) mice.