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. 2020 Jan 28;10:2968. doi: 10.3389/fmicb.2019.02968

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Copyright © 2020 Moreira, Oliveira, Silva, Inácio, Bailão, Parente-Rocha, Cruz-Leite, Paccez, de Almeida Soares, Weber and Borges.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electrophoretic profile of affinity chromatography. One-dimensional (1-D-SDS-PAGE) gel of proteins eluted from Sepharose beads-IgG. Immunoprecipitation using serum from immunized mice (At + Ab) and negative control (C−). Top row: Isolated representatives of Paracoccidioides species (P. lutzii, P. americana, P. restrepiensis, and P. brasiliensis). The arrows indicate the presence of exoantigens after purification when compared to the negative control. At, antigen; Ab, antibody; IgG HC, immunoglobulin G heavy chain; IgG LC, immunoglobulin G light chain. Electrophoresis stained with Coomassie blue.