Figure 6.

AX enhanced the mitochondrial oxidative respiration and the glycolysis capacity in the C2C12 cells. (A,B) AX enhanced the basal mitochondrial respiration rate and maximal respiratory capacity in C2C12 cells treated with vehicle, or 1 or 5 μM AX, for 24 h, using oxygen consumption rate (OCR) as a readout (n = 6–8 in each treatment)). (C,D) AX also enhanced the glycolysis capacity in the C2C12 cells. This parameter was evaluated with the XF Mito Fuel Flex Test kit (Agilent/Seahorse) using the XFe96 Extracellular Flux Analyzer (n = 11 in each treatment). All values are presented as the means ± S.E.M. #p < 0.05, ##p < 0.01, ###p < 0.001 (vehicle vs. AX treatment). Statistical tests one‐way ANOVA/Dunnett's test was performed.