Figure 1.

Repletion of vitamin D attenuates cachexia in Ctns^−/− mice. Twelve‐month‐old wild‐type (WT) and Ctns^−/− mice were treated with 25(OH)D₃ and 1,25(OH)₂D₃ (75 μg/kg/day and 60 ng/kg/day, respectively) or ethylene glycol as vehicle for 6 weeks. Four groups of mice were included: WT + vehicle (n = 8), WT + 25(OH)D₃ + 1,25(OH)₂D₃ (n = 8), Ctns^−/− + vehicle (n = 8), and Ctns^−/− + 25(OH)D₃ + 1,25(OH)₂D₃ (n = 8). Ctns^−/− + vehicle mice were fed ad libitum, whereas other groups of mice were fed the same amount of food intake as that of Ctns^−/− + vehicle mice. Data are expressed as mean ± standard error of the mean. ^# P < 0.05, significantly different in Ctns^−/− + vehicle and Ctns^−/− + 25(OH)D₃ + 1,25(OH)₂D₃ mice vs. WT + vehicle and WT + 25(OH)D₃ + 1,25(OH)₂D₃ mice, respectively. Results of Ctns^−/− + vehicle mice were also compared with Ctns^−/− + 25(OH)D₃ + 1,25(OH)₂D₃ mice.