Table 1.

Serum and blood chemistry of mice

	WT + vehicle	WT + 25(OH)D3 + 1,25(OH)2D3	Ctns−/− + vehicle	Ctns−/− + 25(OH)D3 + 1,25(OH)2D3
	n = 8	n = 8	n = 8	n = 8
BUN (mg/dL)	32.4 ± 5.5	32.5 ± 3.8	79.6 ± 14.6a	80.2 ± 9.8a
Ca (mg/dL)	11.2 ± 0.6	11.3 ± 0.8	9.1 ± 0.5b	10.6 ± 0.4
Creatinine (mg/dL)	<0.2 ± 1.1	0.3 ± 0.2	0.7 ± 0.2a	0.8 ± 0.2a
Bicarbonate (mmol/L)	27.8 ± 0.5	27.6 ± 1.7	26.8 ± 1.1	26.7 ± 2.4
Pi (mg/dL)	7.5 ± 0.5	7.6 ± 0.3	9.8 ± 0.4a	8.2 ± 0.5c
PTH (pg/mL)	108.4 ± 9.4	118.6 ± 18.4	364.1 ± 21.4a	227.3 ± 17.5a,c
25(OH)D3 (ng/mL)	113.2 ± 12.3	103.7 ± 23.4	43.5 ± 15.4b	117.8 ± 15.3c
1,25(OH)2D3 (pg/mL)	298.4 ± 23.4	278.4 ± 26.9	105.6 ± 24.8b	302.4 ± 37.8c

BUN, blood urea nitrogen; PTH, parathyroid hormone; WT, wild‐type.

Twelve‐month‐old, male, WT, and Ctns^−/− mice were treated with 25(OH)D₃ and 1,25(OH)₂D₃ (75 μg/kg/day and 60 ng/kg/day, respectively) or ethylene glycol as vehicle for 6 weeks. Four groups of mice were included: WT + vehicle, WT + 25(OH)D₃ + 1,25(OH)₂D_3, Ctns^−/− + vehicle, and Ctns^−/− + 25(OH)D₃ + 1,25(OH)₂D₃. Ctns^−/− + vehicle mice were fed ad libitum, while other groups of mice were fed the same food intake as that of Ctns^−/− + vehicle mice. Data are expressed as mean ± standard error of the mean.

^a P < 0.05, significantly higher in Ctns^−/− + vehicle and Ctns^−/− + 25(OH)D₃ + 1,25(OH)₂D₃ mice vs. WT + vehicle and WT + 25(OH)D₃ + 1,25(OH)₂D₃ mice, respectively.

^b P < 0.05, significantly lower in Ctns^−/− + vehicle and Ctns^−/− + 25(OH)D₃ + 1,25(OH)₂D₃ mice vs. WT + vehicle and WT + 25(OH)D₃ + 1,25(OH)₂D₃ mice, respectively.

^c P < 0.05, significantly different between Ctns^−/− + vehicle vs. Ctns^−/− + 25(OH)D₃ + 1,25(OH)₂D₃ mice.