Fig 5. Additional challenge with CD99 leads to increased autoantibody levels.

Mice were treated as described in Fig 4. Sera from mice were subjected to protein microarray analysis containing 192 antigens. (A) Changes in autoantibody levels depicted as volcano plots. A Student t- Test was performed to discriminate between the groups and changes with a trend to significance (p<0.1) are depicted in blue. (B) Changes in anti CD99 antibody expression levels depicted as a boxplot diagram. Sample size: Control n = 10, Ethanol + PBS n = 16, Ethanol + CD99 n = 13. Boxes represent upper and lower quartiles, whiskers represent variability and outliers are plotted as individual points. For comparison of groups, ANOVA followed by Tukey’s HSD was conducted.