Table 1. Expression levels of CEACAM5, KLK6, SLC35D3, MUC2, and POSTN mRNAs in primary CRC tumors, normal colon, normal colon epithelial cells, CC cell lines, peripheral blood mononuclear cells, immune cell lines, a fibroblast cell line, CC liver metastases and normal liver.

			mRNA copies / 18S rRNA unit
SOURCE		n°	CEACAM5	KLK6	SLC35D3	MUC2	POSTN
Primary CRC		57	176*	2.9	0.1	1.1	9.7
tumors			(112–281)**	(0.9–8.5)	(0.04–0.4)	(0.3–4.2)	(4.6–22.2)
CC cell	LS174T	1	328#	79	0##	4.3	0
lines	HT29	1	32	256	0.02	0.01	0
	T84	1	33	316	0.7	0.5	0
	HCT8	1	32	32	0.07	0.02	0
	CaCo2	1	3	0.4	0.09	0.04	0.0009
Normal	Tissue	5	222*	0	0.02	9	5.9
colon	iECs	5	300*	0	0.0009	32	0.2
PBMCs	Fresh	1	0	0	0.06	0	0
	Act.	1	0	0	0	0	0
Immune cell lines
T cell	Jurkat	1	0	0	0	0	0.009
B cell	CNB6 + KR4	1	0	0	0	0	0
Plasma cell	U266	1	0	0	0	0	0.005
Monocyte	U937	1	0.005	0	0	0	0
Granulocyte	HL60	1	0	0	0	0	0
Other cell lines
Erythroid	K562	1	0	0	0.09	0	0.001
Fibroblasts	FSU	1	0.0002	0	0	0.004	5.5
Liver tissue
Metastasis		2	78	2	0.07	0.003	22.6
Normal		2	0.01	0	0	0.00004	2.3

° n = number of samples.

* Median of indicated number of samples.

** Interquartile range from the 25^th to the 75^th percentile.

^# Cell lines and PBMCs: mean of 3 independent determinations.

^## 0, < 0.00001 mRNA copies/18S rRNA unit.

iECs, isolated epithelial cells; PBMCs, peripheral blood mononuclear cells. The T-stages of the 57 primary tumors were: T2, 11; T3, 39; T4, 7.