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. 2020 Jan 31;16(1):e1007643. doi: 10.1371/journal.pcbi.1007643

Fig 3. Multi-gene expression in single-cells during transcriptional competition.

Fig 3

(A) Transcriptional competition between car-mCherry and gfp genes for limited shared RNAPs in single cells. CAR-mCherry mRNA and GFP mRNA were hybridized by Quasar 670- (blue) and Quasar 570-labeled (red) probes, respectively. The fluorescence of the mCherry protein was deactivated via the M71G mutation to prevent spectral overlap. (B) Representative FISH images of single cells induced at 500 μM IPTG. (C) Population mean mRNA copy numbers of GFP and CAR-mCherry at various IPTG concentrations. mRNA copy numbers of CAR-mCherry and GFP were estimated from fluorescence intensity. Error bars represent the 95% confidence interval, determined by bootstrapping. (D) GFP and CAR-mCherry mRNA copy numbers of single cells at various IPTG induction levels.