Fig 4. Polycistronic operon enables highly correlated protein expression.
(A) Various promoters are used to control the co-expression of mCherry and GFP from a polycistronic operon. (B) mCherry and GFP in individual cells under the control of the inducible promoter PlacUV5 at different IPTG induction levels. a.u., arbitrary units. (C) mCherry and GFP in individual cells under the control of constitutive promoters with different strengths. (D) Relationships among variability, mean, and correlation between mCherry and GFP in the inducible promoter construct. (E) Relationships among variability, mean, and correlation between mCherry and GFP in promoter library constructs. Variability and mean are quantified using GFP.