Figure 1.

Methylation sites measured and timeline of sample collection. A. Each assay measures a different methylation signature in the INS gene. Maroon open circles are CpG sites. Assay M (green arrow) measures one site in the promoter region at –69. Assay H (orange bracket) measures 2 CpGs in Exon 2, +396 and +399. Assay D (blue bracket) measures 6 CpGs in Exon 2, from +303. B. Supernatant from islet transfer IV bag was collected just before islet infusion. Serum and plasma were collected at timepoints postpancreatectomy but before islet infusion, as well as 15 to 30 minutes and 3 days posttransplant. Urine was collected prior to islet transplant but after pancreatectomy and at 5 hours postpancreatectomy.