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Bisulfite PCR primers and products
| Assay D | Assay H | Assay M | |
|---|---|---|---|
| Forward primer | TTTTTGGGGATTTGATTTAGT | GTGGTTTATATTTGGTGGA | GGAAATTGTAGTTTTAGTTTTTAGTTATTTGT |
| Reverse primer | ACCCTACAAATCCTCTACCTCC | ATTAACTCACCCTACAAATC | AAAACCCATCTCCCCTACCTATCA |
| Unmethylated fragment probe | ATTTAAGATTTGTTGGGAGGTAGAG | ACCCCTACCACCTAAC | |
| Methylated fragment probe | ATTTAAGATTCGTCGGGAGGTAGAG | ACCCCTACCGCCTAAC | |
| Product size | 144 base pairs | 106 base pairs | 157 base pairs |
