Figure 1.

Schematic representation of available data for four different hypothetical compounds. They belong to the same type of compound; for example, belonging to the same type of fungicide. All four compounds showed anti‐androgenic activity in vitro. Three compounds have an extended one‐generation study showing reduced anogenital distance of male rats, whereas the fourth compound lacks this study. The question is whether this study should be requested for the fourth compound? In such a situation, a read‐across hypothesis supported on AOP‐based testing for the effect could possibly answer the question and thus an in vivo study would be obsolete

• +: Available data
• ↓: Activity decreased
• n.a.: No activity