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. 2020 Jan 17;128(1):015001. doi: 10.1289/EHP6104

Figure 2.

Figure 2 is a scatterplot, plotting scalability across direct biological relevance to compare major techniques for epigenetics analyses against a hypothetical, ideal technique for screening that is highly scalable and biologically relevant, marking in silico methods (molecular docking); global methods (in vitro reporters, repetitive element sequencing, ELISA, HPLC, mass spectrometry, and dot blots; locus-specific methods include in vitro reporters, amplicon sequencing, bisulfite pyrosequencing, in vivo reporters, and IAP animal models); epigenome-wide methods include miRNA arrays, methylation arrays, RRBS, histone ChIP-seq, and WGBS; and higher order chromatin structure assessments include super-resolution imaging and ATAC-seq.

Comparison of major techniques for epigenetics analyses on subjective scales for scalability and direct biological relevance. Axes are in arbitrary scales, with scalability denoting how amenable a given technique is to be implemented in a high-throughput screening setting. Direct biological relevance denotes how information-rich the results of a given technique are. Note: ATAC-seq, assay for transposase-accessible chromatin using sequencing; ChIP, chromatin immunoprecipitation sequencing; ELISA, enzyme-linked immunosorbent assay; HPLC, high-performance liquid chromatography; RRBS, reduced-representation bisulfite sequencing; WGBS, whole-genome bisulfite sequencing.