Table 1.

Primers used for RT-PCR in this study.

Gene	Primer sequence 5ʹ–3ʹ
Occludin (Human)	(F) CTG AAG TGG TTC AGG AGC TTC CAT (R) CTT TGA CCT TCC TGC TCT TCC CTT
Claudin-1 (Human)	(F) AAG ATG AGG ATG GCT GTC ATT GGG (R) CAT TGA CTG GGG TCA TAG GGT CAT
MMP-9 (Human)	(F) CCT CGA ACT TTG ACA GCG ACA AGA (R) CTC CGG CAC TGA GGA ATG ATC TAA
MLCK (Human)	(F) CTC AGT GGC AGG AAA TCC TCA ACA (R) CAT GAG GCT TTT CCT CAG CAA CAG
$\text{TNF-}\mathrm{\alpha }$ (Human)	(F) AAG CCC TGG TAT GAG CCC ATC TAT (R) AGG GCA ATG ATC CCA AAG TAG ACC
$\text{IL-}1\mathrm{\beta }$ (Human)	(F) TAC CTG AGC TCG CCA GTG AAA T (R) CCT GGA AGG AGC ACT TCA TCT GTT
IL-6 (Human)	(F) ACA GCC ACT CAC CTC TTC AGA AC (R) TTT TCT GCC AGT GCC TCT TTG C
GAPDH (Human)	(F) GAC CCC TTC ATT GAC CTC AAC TAC (R) ATG ACA AGC TTC CCG TTC TCA G
$\text{TNF-}\mathrm{\alpha }$ (Mouse)	(F) AGG GAT GAG AAG TTC CCA AAT GGC (R) GGC TAC AGG CTT GTC ACT CGA AT
$\text{IL-}1\mathrm{\beta }$ (Mouse)	(F) TTG ACG GAC CCC AAA AGA TGA AGG (R) ACA GCT TCT CCA CAG CCA CAA T
IL-6 (Mouse)	(F) TTC TTG GGA CTG ATG CTG GTG A (R) TTA AGC CTC CGA CTT GTG AAG TGG
GAPDH (Mouse)	(F) AAC TCC CAC TCT TCC ACC TTC GAT (R) CCC TGT TGC TGT AGC CGT ATT CAT

Note: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; MLCK, myosin light-chain kinase; MMP, metallopeptidase; RT-PCR, real-time polymerase chain reaction; TNF, tumor necrosis factor.