Figure 13.

De novo synthesis of cholesterol and sterol precursors was accomplished by incubating hiPSC-derived neuroprogenitor cells with ${}^{13}{\mathrm{C}}_3\text{-lanosterol}$ for 24 h and monitoring for ${}^{13}{\mathrm{C}}_3\text{-sterols}$ at indicated time points. Isotopically labeled sterols analyzed are lanosterol (solid line), desmosterol (solid square, dashed line), 8-DHC (open triangle, solid line), 7-DHC (solid circle, solid line), and cholesterol (open circle, dashed line). Three distinct differentiations were conducted for each donor and averaged; shown is the average of the three donors $\pm \text{SEM}$ ($n=3$). *$p<0.05$, **$p<0.01$, ***$p<0.001$ as determined by repeated measures one-way analysis of variance (ANOVA).