Figure 1.

Body weight, white adipose tissue weight, adipose magnetic resonance imaging (MRI), and liver lipid of male and female mice developmentally exposed to tributyltin or vehicle. (A) Female mice were fed a phytoestrogen-free diet and treated with tributyltin (TBT) in their drinking water beginning 2 weeks prior to breeding lasting through gestation and lactation. Offspring were then weaned at 3 weeks of age and fed the same phytoestrogen-free diet until euthanasia at 14, 20, or 45 weeks of age. (B) Body weights, white adipose tissue depot [either inguinal (iWAT) or scapular (scWAT)], and brown adipose tissue (BAT) wet weights in 14-week-old animals developmentally exposed to TBT or VEH are displayed; VEH-treated females, $n=4$ (from two litters with two pups per litter); VEH-treated males, $n=3$ (from two litters); TBT-treated females, $n=3$ (from two litters); TBT-treated males, $n=4$ (from two litters, two pups per litter). (C) Representative full-body MRI scans are presented on the left and quantitation of surface area is presented on the right for 20-week-old animals: for VEH-treated females, $n=2$ (from one litter); for TBT-treated females, $n=4$ (from two litters with two pups per litter); for VEH-treated males, $n=2$; and for TBT-treated males, $n=4$. (D) Macroscopic images of liver tissue at 20 weeks, and Oil Red O stained, left liver lobes are presented on the left with quantitation of Oil Red O staining on the right, $n=6$ for VEH-treated males, $n=10$ for TBT-treated males, $n=7$ for VEH-treated females, and $n=9$ for TBT-treated females. Scale bars: $100\mathrm{\mu }\mathrm{m}$. In all graphs, VEH bars are hatched and TBT bars are solid, all bars represent the $\text{mean}\pm \text{standard}$ error of the mean (SEM) and t-tests were performed to ascertain significance (*$p<0.05$). Excel Table S1 details information on n and litter. Note: ORO, Oil Red O.