Figure 4.

Differential gene expression in tributyltin (TBT)-exposed liver samples and overlap with adenoma signatures. (A) Upper, hierarchical clustering of RNA-seq data from liver samples from 20- and 45-week-old male mice using differentially expressed genes [false discovery rate (FDR) adjusted $p<0.05$ with a fold change exceeding $2\times$]. Lower, principal component analysis of RNA-seq data at each time point. Triangles represent vehicle (VEH)-exposed liver samples, and circles represent TBT-exposed liver samples. (B) Venn diagrams of overlapping genes in all four gene expression signatures (up-regulated–left, down-regulated–right). A $\left(\text{pink}\right)=\text{Adenoma}$ ($n=7$) over TBT-exposed liver ($n=5$); B $\left(\text{blue}\right)=\text{Adenoma}$ ($n=7$) over VEH-exposed liver ($n=6$); C $\left(\text{yellow}\right)=\text{TBT-exposed \hspace{0.17em} liver}$ ($n=5$) over VEH-exposed liver ($n=6$) at 20 weeks; and D $\left(\text{green}\right)=\text{TBT-exposed \hspace{0.17em} liver}$ ($n=5$) over VEH-exposed liver ($n=6$). See Excel Table S1 for detail information on n and litter.