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. Author manuscript; available in PMC: 2020 Feb 12.
Published in final edited form as: Anal Chem. 2019 Jun 18;91(13):8615–8621. doi: 10.1021/acs.analchem.9b01954

Figure 7.

Figure 7.

Time-course for the production of 2TA upon incubation of a B. subtilis cell lysate and Tb3+ with ester 2. Emission intensity was recorded in 5 mM HEPES–NaOH buffer, pH 7.4, containing ester 2 (50 or 100 μM), TbCl3 (4 mM), lysate, Triton X-100 (0.1% w/v), and TOPO (350 μM).