Skip to main content
. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: J Invest Dermatol. 2018 Nov 16;139(5):1118–1126. doi: 10.1016/j.jid.2018.10.038

Figure 6. Blocking collagen prolyl 4-hydroxylase inhibits melanoma invasion.

Figure 6.

(a) EDHB inhibits invasiveness of PMWK 3-dimensional tumor spheroids. Representative images from two independent experiments performed in triplicate. Spheroids were imaged at ×2 on days 0, 3, and 6 post treatment.(b) Quantification of the effect of EDHB on PMWK 3-dimensional spheroid invasion. Spheroids were imaged at ×2 on days 0, 3, and 6 post treatment. Data are mean ± standard error of the mean (n = 2) performed in triplicate. Significance was compared to the appropriate control using two-way analysis of variance with Tukey’s post-hoc testing. (c) Overexpression of C-P4H genes is associated with worse disease-free survival in melanoma. Kaplane–Meier plots by gene expression were created using the “Tumor Melanoma-Jönsson-214-custom-ilmnht12v4” data set (n = 214) (Cirenajwis et al., 2015). EDHB, ethyl dihydroxybennzoic acid.