Table 2. Cryo-EM KLp61F motor and tail MT structures: collection and reconstruction.
| Dm Klp61F motor-AMPPNP (15 protofilaments) |
Dm Klp61F- motor AMPPNP (14 protofilaments) |
Dm KLP61F motor-tail- nucleotide free (15 protofilaments) |
Dm Klp61F5 motor-tail-nucleotide free (14-protofilaments) |
|
|---|---|---|---|---|
| Data collection | ||||
| Microscope | Titan Krios (FEI) | Titan Krios (FEI) | Titan Krios (FEI) | Titan Krios (FEI) |
| Voltage (kV) | 300 | 300 | 300 | 300 |
| Ls | 22,500X | 22,500X | 22,500X | 22,500X |
| Cumulative exposure dose (e- Å−2) | 38 | 38 | 40 | 40 |
| Exposure rate (e-/pixel/sec) | 7.9 | 7.9 | 8.3 | 8.3 |
| Detector | K2 Summit | K2 Summit | K2 Summit | K2 Summit |
| Pixel size (Å)* | 1.31 | 1.31 | 1.31 | 1.31 |
| Defocus range (µm) | 0.3–3.78 | 0.7–3.78 | 0.19–5.12 | 0.19–5.12 |
| Average defocus (m) | 1.75 | 1.75 | 1.86 | 1.86 |
| Micrographs Used | 1260 | 1260 | 955 | 955 |
| Total extracted helical segment (no.) | 73,451 | 73,451 | 44,081 | 44,081 |
| Refined helical segment (no.) | 21,004 | 39,001 | 9490 | 27,433 |
| Reconstruction | ||||
| Final helical segments (no.) | 21,004 | 39,220 | 9490 | 14,570 |
| Symmetry imposed | HP | HP | HP | HP |
| Resolution (global) FSC 0.143 | 4.2 | 4.4 | 4.2 | 4.3 |