Table 3. Cryo-EM refinement and Structure model statistics.
| Dm-Klp61F motor AMPPNP-MT | Dm-Klp61F motor -Nucleotide free-MT | |
|---|---|---|
| Data collection | ||
| Microscope/detector | Titan Krios/Gatan K2 | Titan Krios/Gatan K2 |
| Magnification | 22,500x | 22,500x |
| Voltage (keV) | 300 | 300 |
| Dose rate (electrons/pixel/second) | 7.96 | 8.3 |
| Pixel size (Å/pixel) | 1.31 | 1.31 |
| Map resolution (Å) | 4.4 | 4.4 |
| FSC threshold | 0.143 | 0.143 |
| Refinement | ||
| Model resolution cutoff (Å) | 4.4 | 4.4 |
| FSC threshold | 0.143 | 0.143 |
| Protein residues | 1173 | 1174 |
| Ligands | 3 (GTP/GDP/AMPPNP) | 2 (GTP/GDP) |
| Map CC | 0.8 | 0.78 |
| B factor (Å) | 216 | 208 |
| R.M.S deviations | ||
| Bond lengths (Å) | 0.003 | 0.006 |
| Bond angles (°) | 0.53 | 1.14 |
| Validation | ||
| All-atom clash score | 14.05 | 11.71 |
| MolProbity score | 2.52 | 1.9 |
| Ramachandran plot | ||
| Favored (%) | 96.19 | 94.65 |
| Allowed (%) | 3.81 | 5.18 |
| Outliers (%) | 0.00 | 0.17 |