Table 5. Motor motility and MT sliding parameters in vitro MT sliding assays.

Single motor velocities in relation to free MT sliding motility
FL-Eg5-GFP	Free MT sliding motility (nm/s)	Motility in sliding zones (nm/s)
25 mM KCl	13.8 ± 1.0 n = 26	13.9 ± 1.0 n = 32
50 mM KCl	31.2 ± 1.2 n = 33	22.7 ± 1.2 n = 71
Single motor motility within MT sliding zones
	Eg5-Δtail-GFP motors (nm/s)	FL-Eg5-GFP motors (nm/s)
Overlap Zone	8.6 ± 0.9 n = 32	3.4 ± 0.3 n = 67
Single MT	9.6 ± 0.8 n = 52	5.6 ± 0.3 n = 45