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. 2020 Feb 12;6(7):eaay2387. doi: 10.1126/sciadv.aay2387

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Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 1 — (A) Medium containing cell-secreted ECM was mixed with (B) mononucleated cells from BMA and seeded onto (C) HA–poly(lactide-co-glycolide) (PLG) scaffolds using a perfusion bioreactor. After 20 hours of perfusion, (D) naïve scaffolds were retrieved, and (E) preconditioned scaffolds were perfused with culture medium containing osteogenic supplements for 13 additional days. (F) Scaffolds were implanted into 3.5-mm bilateral calvarial defects.