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. 2020 Feb 12;6(7):eaax3157. doi: 10.1126/sciadv.aax3157

Fig. 2. Undocked hemichannel structures of WT INX-6 in detergent and INX-6ΔN in a nanodisc.

Fig. 2

(A) Sliced views of the Gaussian-filtered 3D density map of WT INX-6 in detergent. The surrounding detergent densities are shown in pink, and the N-terminal funnels are shown in red. The ribbon style model is superimposed. The map contour level is 1.5σ. (B) Ribbon style model of a monomeric structure of WT INX-6 in detergent. The assigned residues at the N terminus begin from N12 (shown in stick style), and the I52 and G53 residues are not visible due to disorder. (C) Sliced views of the Gaussian-filtered 3D density map of INX-6ΔN in a nanodisc. The densities corresponding to the nanodisc are shown in magenta, and the ribbon style model is superimposed. The double-layer densities in the pore are shown in green. The thickness of the top view slab is indicated by the bracket in the side view. The map contour level is 1.5σ. (D) Comparison of WT INX-6 to INX-6ΔN in nanodiscs. A subtraction map of INX-6ΔN from WT INX-6 (blue) is superimposed on the structure of INX-6ΔN (yellow), where the represented slab corresponds to the bracket in (C). The map contour level is 3σ.