The pupal duration was quantified in wild type Canton S, Mhc1/CyO; Mef2GAL4/UAS-Mhcwt, Mhc1/CyO; Mef2GAL4/UAS-MhcR672H, Mhc1/CyO; Mef2GAL4/UAS-MhcR672C and Mhc1/CyO; Mef2GAL4/UAS-MhcT178I flies (A). Climbing assay to test muscle function was carried out at 5 (B) and 20 (C) days post-eclosion using wild type Canton S, Mhc1/CyO; Mef2GAL4/UAS-Mhcwt, Mhc1/CyO; Mef2GAL4/UAS-MhcR672H, Mhc1/CyO; Mef2-GAL4/UAS-MhcR672C and Mhc1/CyO; Mef2GAL4/UAS-MhcT178I flies respectively. Number of flies that climbed 8 cm or above, 4–8 cm or 0–4 cm were counted, and the percentage of each category is represented as the climbing index. The graphical data is presented as mean ± standard error of the mean.