FIG 3.

Characterization of the three different genotypes of A. aromaticum EbN1. (A) (Bottom) Wild type, ΔpcrSR mutant, and pcrSR-complemented mutant were initially cultivated with a growth-limiting supply of benzoate (1 mM). Following depletion of benzoate, p-cresol was added to each culture (red dashed lines), yielding a final concentration of 100 μM. (Top, enlargements from dashed-line boxes) Subsequently, samples for targeted transcript analysis were taken in a time-resolved manner (gray dashed lines). (B) Transcript analysis (qRT-PCR) of the denoted target genes. Data points were connected with LOESS (locally estimated scatterplot smoothing) regression curves, with shaded bands indicating 95% confidence intervals. (C) Increased transcript abundances of the signaling system components in the pcrSR-complemented mutant relative to their levels in the wild type. (D) MS-based protein identification, with numbers representing Mascot scores. n.d., not detected.