FIG 6.

ROS accumulation and oxidative stress assays in ΔmntR and Δzur mutants. (A) Quantification of O₂^·− produced in wild-type (WT), ΔmntR, and Δzur cells at an OD₆₀₀ of 1 in LB medium. (B) Quantification of H₂O₂ produced in WT, ΔmntR, and Δzur cells during 4 h of growth in LB medium. (C) Plates from a disk diffusion assay with a drop of 3 M hydrogen peroxide (H₂O₂). (D) The histograms represent the percentage of survival of WT, ΔmntR, and Δzur cells at 15 min after addition of 0.01, 0.05, or 0.1 mM H₂O₂. The survival rate was determined as the ratio of the number of CFU per milliliter after 15 min to the number of CFU per milliliter before addition of H₂O₂.