TABLE 3.
Proteins differentially abundant in the B. subtilis ΔmntR mutant
| Protein | Fold change in abundance in ΔmntR vs wild type | Gene | Function | Functional categorya |
|---|---|---|---|---|
| Proteins upregulated in cytosolic fraction | ||||
| DhbBb | 12.75 | dhbB | Isochorismatase | Acquisition of iron |
| CitB | 10.5 | citB | Aconitase | TCA cycle |
| BglH | 9.25 | bglH | Phospho-beta-glucosidase | Utilization of salicin |
| Spo0M | 9.25 | spo0M | Sporulation | Sporulation |
| PgcA | 8.25 | pgcA | Alpha-phosphoglucomutase | Biosynthesis of teichoic acid |
| RocA | 7.25 | rocA | 3-Hydroxy-1-pyrroline-5-carboxylate dehydrogenase | Arginine utilization/nitrogen metabolism |
| RbsK | 7 | rbsK | Ribokinase | Utilization of ribose |
| MntAb | 6.75 | mntA | Manganese ABC transporter | Acquisition of manganese |
| Syv | 6.5 | valS | Valyl-tRNA synthetase | Translation |
| Mao4 | 6.25 | ytsJ | NADP-dependent malate dehydrogenase | Utilization of malate |
| Syh | 5.75 | hisS | Histidyl-tRNA synthetase | Translation |
| RocGb | 5.25 | rocG | Glutamate dehydrogenase | Arginine utilization/nitrogen metabolism |
| MenB | 5.25 | menB | Naphthoate synthase | Biosynthesis of menaquinone |
| ZagA | 5.25 | zagA | Zinc metallochaperone | Acquisition of zinc |
| Proteins upregulated in membrane fraction | ||||
| DhbFb | 29 | dhbF | Involved in bacillibactin biosynthesis | Acquisition of iron |
| MntAb | 17.5 | mntA | Manganese ABC transporter | Acquisition of manganese |
| MtnK | 8.25 | mtnK | 5-Methylthioribose kinase | Methionine metabolism |
| RocGb | 7.5 | rocG | Glutamate dehydrogenase | Arginine utilization/nitrogen metabolism |
| MntB | 6.25 | mntB | Manganese ABC transporter | Acquisition of manganese |
| DnaX | 5.5 | dnaX | DNA polymerase III | DNA replication |
| Proteins downregulated in cytosolic fraction | ||||
| RplD | −25.5 | rplD | Ribosomal protein L4 | Translation |
| CarB | −22.5 | carB | Carbamoyl-phosphate transferase-arginine | Biosynthesis of arginine |
| CspB | −20.5 | cspB | RNA chaperone | Cold stress proteins |
| FabI | −20.5 | fabI | Enoyl-acyl carrier protein reductase | Fatty acid biosynthesis |
| YpfD | −11.5 | ypfD | Similar to ribosomal protein S1 | Translation |
| SodM | −11 | sodA | Superoxide dismutase | Resistance against oxidative stress |
| YkaA | −9.75 | ykaA | Unknown | Proteins of unknown function |
| PunA | −8.75 | pupG | Purine nucleoside phosphorylase | Nucleotide metabolism |
| RpsM | −8.25 | rpsM | Ribosomal protein S13 | Translation |
| OppA | −7.25 | oppA | Oligopeptide ABC transporter | Utilization of peptides |
| PtsG | −6.5 | ptsG | Glucose permease | Carbon core metabolism |
| Hbs | −6.25 | hbs | Nonspecific DNA-binding protein | DNA condensation/segregation |
| AsnB | −6 | asnB | Asparagine synthase | Control of peptidoglycan hydrolysis |
| PbpC | −6 | pbpC | Penicillin-binding protein 3 | Cell wall synthesis |
| Protein downregulated in membrane fraction: FlgG | −5.75 | flgE | Flagellar hook protein | Motility and chemotaxis |
a
According to the SubtiWiki database (77).
b
Protein detected in both cytosolic and membrane fractions.