TABLE 4.
Protein | Fold change in abundance in Δzur vs WT | Gene | Function | Functional categorya |
---|---|---|---|---|
Proteins upregulated in cytosolic fraction | ||||
ZagAb | 243 | zagA | Zinc metallochaperone | Acquisition of zinc |
AdcAb | 34 | znuA | ABC transporter for zinc | Acquisition of zinc |
FolE2b | 22.25 | folE2 | GTP cyclohydrolase IB | Biosynthesis of folate |
CarB | 18 | carB | Carbamoyl-phosphate transferase-arginine | Biosynthesis of arginine |
DnaK | 15.5 | dnaK | Molecular chaperone | Protein quality control |
RocA | 14.25 | rocA | 3-Hydroxy-1-pyrroline-5-carboxylate dehydrogenase | Arginine utilization/nitrogen metabolism |
AcoN | 10.5 | citB | Aconitase | TCA cycle |
ZinTb | 8 | zinT | Zinc-binding protein | Acquisition of zinc |
GlmU | 7.75 | glmU | Bifunctional N-acetylglucosamine-1-phosphate | Biosynthesis of peptidoglycan |
RbsK | 7.25 | rbsK | Ribokinase | Utilization of ribose |
PgcA | 7.25 | pgcA | Alpha-phosphoglucomutase | Biosynthesis of teichoic acid |
Syi | 7.25 | ileS | Isoleucyl-tRNA synthetase | Translation |
Fbp | 7 | fbp | Fructose-1,6-bisphosphatase | Gluconeogenesis |
GndA | 7 | gndA | NADP-dependent phosphogluconate dehydrogenase | Pentose phosphate pathway |
Oatb | 7 | rocD | Ornithine transaminase | Ornithine utilization/nitrogen metabolism |
PyrGb | 6.75 | pyrG | CTP synthase | Nucleotide metabolism |
RocGb | 6.25 | rocG | Glutamate dehydrogenase | Arginine utilization/nitrogen metabolism |
AdcCb | 5.75 | znuC | ABC transporter for zinc | Acquisition of zinc |
Rl31B | 5.5 | rpmE2 | Accessory ribosomal protein | Translation |
Sya | 5.5 | alaS | Alanine-tRNA synthetase | Translation |
Proteins upregulated in membrane fraction | ||||
ZagAb | 184.75 | zagA | Zinc metallochaperone | Acquisition of zinc |
AdcAb | 100.75 | znuA | ABC transporter for zinc | Acquisition of zinc |
YjlD | 29.25 | ndh | NADH dehydrogenase | Respiration |
GlpK | 28.25 | glpK | Glycerol kinase | Utilization of glycerol |
MetK | 22.75 | metK | S-Adenosylmethionine synthetase | Methionine metabolism |
ZinTb | 19.25 | zinT | Zinc-binding protein | Acquisition of zinc |
AdcCb | 16.75 | znuC | ABC transporter for zinc | Acquisition of zinc |
WapA | 15.5 | wapA | Cell wall-associated protein precursor | Toxins, antitoxins, and immunity against toxins |
AsnB | 14.75 | asnB | Asparagine synthase | Control of peptidoglycan hydrolysis |
FolE2b | 12.25 | folE2 | GTP cyclohydrolase IB | Biosynthesis of folate |
PyrGb | 11 | pyrG | CTP synthase | Nucleotide metabolism |
CadA | 10 | cadA | Cadmium transporting ATPase | Resistance against toxic metals |
YciB | 8.5 | yciB | Putative l,d-transpeptidase | Acquisition of zinc |
Oatb | 7.75 | rocD | Ornithine transaminase | Ornithine utilization/nitrogen metabolism |
MurG | 6.75 | murG | Peptidoglycan precursor biosynthesis | Biosynthesis of peptidoglycan |
RocGb | 6.75 | rocG | Glutamate dehydrogenase | Arginine utilization/nitrogen metabolism |
Smc | 6.25 | smc | Segregation of replication origins | DNA condensation/segregation |
DnaN | 5.5 | dnaN | DNA polymerase III | DNA replication |
Proteins downregulated in cytosolic fraction | ||||
Rl7 | −46.75 | rplL | Ribosomal protein L12 | Translation |
CspB | −37.25 | cspB | RNA chaperone | Cold stress proteins |
Tkt | −25.75 | tkt | Transketolase | Pentose phosphate pathway |
Rs6 | −10.5 | rpsF | Ribosomal protein S6 | Translation |
RpsM | −10 | rpsM | Ribosomal protein S13 | Translation |
Hbs | −9 | hbs | Nonspecific DNA-binding protein | DNA condensation/segregation |
YqiW | −7 | yqiW | Bacilliredoxin | Resistance against oxidative stress |
YjlCb | −7 | yjlC | Unknown | Protein of unknown function |
YxkC | −6.5 | yxkC | Unknown | Protein of unknown function |
WapI | −6.25 | wapI | Immunity protein | Toxins, antitoxins, and immunity against toxins |
PthP | −6 | ptsH | Phosphotransferase system-dependent sugar transport and carbon catabolite repression | Transporters |
Rl15 | −6 | rplO | Ribosomal protein L15 | Translation |
YceD | −5.75 | yceD | Similar to tellurium resistance protein | Resistance against toxic metals |
Rl10 | −5.5 | rplJ | Ribosomal protein L10 | Translation |
Rs19 | −5.5 | rpsS | Ribosomal protein S19 | Translation |
YqeY | −5.25 | yqeY | Unknown | Protein of unknown function |
Proteins down-regulated in membrane fraction | ||||
Fla | −85 | hag | Flagellin protein | Motility and chemotaxis |
Rs8 | −18.25 | rpsH | Ribosomal protein S8 | Translation |
CheA | −8.5 | cheA | Two-component sensor kinase | Motility and chemotaxis |
YjlCb | −8 | yjlC | Unknown | Protein of unknown function |
AtpF | −7.5 | atpF | ATP synthase | ATP synthesis |
FliL | −6.5 | fliL | Flagellar protein | Motility and chemotaxis |
LiaH | −6 | liaH | Phage shock-like protein | Resistance against oxidative stress and cell wall antibiotics |
Spo0M | −6 | spo0M | Unknown | Sporulation |
FlgG | −5.5 | flgE | Flagellar hook protein | Motility and chemotaxis |
GgaB | −5 | ggaB | Membrane protein | Biosynthesis of teichoic acid |
According to the SubtiWiki database (77).
Protein detected in both cytosolic and membrane fractions.