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. 2020 Feb 11;202(5):e00547-19. doi: 10.1128/JB.00547-19

TABLE 4.

Proteins differentially abundant in the B. subtilis Δzur mutant

Protein Fold change in abundance in Δzur vs WT Gene Function Functional categorya
Proteins upregulated in cytosolic fraction
    ZagAb 243 zagA Zinc metallochaperone Acquisition of zinc
    AdcAb 34 znuA ABC transporter for zinc Acquisition of zinc
    FolE2b 22.25 folE2 GTP cyclohydrolase IB Biosynthesis of folate
    CarB 18 carB Carbamoyl-phosphate transferase-arginine Biosynthesis of arginine
    DnaK 15.5 dnaK Molecular chaperone Protein quality control
    RocA 14.25 rocA 3-Hydroxy-1-pyrroline-5-carboxylate dehydrogenase Arginine utilization/nitrogen metabolism
    AcoN 10.5 citB Aconitase TCA cycle
    ZinTb 8 zinT Zinc-binding protein Acquisition of zinc
    GlmU 7.75 glmU Bifunctional N-acetylglucosamine-1-phosphate Biosynthesis of peptidoglycan
    RbsK 7.25 rbsK Ribokinase Utilization of ribose
    PgcA 7.25 pgcA Alpha-phosphoglucomutase Biosynthesis of teichoic acid
    Syi 7.25 ileS Isoleucyl-tRNA synthetase Translation
    Fbp 7 fbp Fructose-1,6-bisphosphatase Gluconeogenesis
    GndA 7 gndA NADP-dependent phosphogluconate dehydrogenase Pentose phosphate pathway
    Oatb 7 rocD Ornithine transaminase Ornithine utilization/nitrogen metabolism
    PyrGb 6.75 pyrG CTP synthase Nucleotide metabolism
    RocGb 6.25 rocG Glutamate dehydrogenase Arginine utilization/nitrogen metabolism
    AdcCb 5.75 znuC ABC transporter for zinc Acquisition of zinc
    Rl31B 5.5 rpmE2 Accessory ribosomal protein Translation
    Sya 5.5 alaS Alanine-tRNA synthetase Translation
Proteins upregulated in membrane fraction
    ZagAb 184.75 zagA Zinc metallochaperone Acquisition of zinc
    AdcAb 100.75 znuA ABC transporter for zinc Acquisition of zinc
    YjlD 29.25 ndh NADH dehydrogenase Respiration
    GlpK 28.25 glpK Glycerol kinase Utilization of glycerol
    MetK 22.75 metK S-Adenosylmethionine synthetase Methionine metabolism
    ZinTb 19.25 zinT Zinc-binding protein Acquisition of zinc
    AdcCb 16.75 znuC ABC transporter for zinc Acquisition of zinc
    WapA 15.5 wapA Cell wall-associated protein precursor Toxins, antitoxins, and immunity against toxins
    AsnB 14.75 asnB Asparagine synthase Control of peptidoglycan hydrolysis
    FolE2b 12.25 folE2 GTP cyclohydrolase IB Biosynthesis of folate
    PyrGb 11 pyrG CTP synthase Nucleotide metabolism
    CadA 10 cadA Cadmium transporting ATPase Resistance against toxic metals
    YciB 8.5 yciB Putative l,d-transpeptidase Acquisition of zinc
    Oatb 7.75 rocD Ornithine transaminase Ornithine utilization/nitrogen metabolism
    MurG 6.75 murG Peptidoglycan precursor biosynthesis Biosynthesis of peptidoglycan
    RocGb 6.75 rocG Glutamate dehydrogenase Arginine utilization/nitrogen metabolism
    Smc 6.25 smc Segregation of replication origins DNA condensation/segregation
    DnaN 5.5 dnaN DNA polymerase III DNA replication
Proteins downregulated in cytosolic fraction
    Rl7 −46.75 rplL Ribosomal protein L12 Translation
    CspB −37.25 cspB RNA chaperone Cold stress proteins
    Tkt −25.75 tkt Transketolase Pentose phosphate pathway
    Rs6 −10.5 rpsF Ribosomal protein S6 Translation
    RpsM −10 rpsM Ribosomal protein S13 Translation
    Hbs −9 hbs Nonspecific DNA-binding protein DNA condensation/segregation
    YqiW −7 yqiW Bacilliredoxin Resistance against oxidative stress
    YjlCb −7 yjlC Unknown Protein of unknown function
    YxkC −6.5 yxkC Unknown Protein of unknown function
    WapI −6.25 wapI Immunity protein Toxins, antitoxins, and immunity against toxins
    PthP −6 ptsH Phosphotransferase system-dependent sugar transport and carbon catabolite repression Transporters
    Rl15 −6 rplO Ribosomal protein L15 Translation
    YceD −5.75 yceD Similar to tellurium resistance protein Resistance against toxic metals
    Rl10 −5.5 rplJ Ribosomal protein L10 Translation
    Rs19 −5.5 rpsS Ribosomal protein S19 Translation
    YqeY −5.25 yqeY Unknown Protein of unknown function
Proteins down-regulated in membrane fraction
    Fla −85 hag Flagellin protein Motility and chemotaxis
    Rs8 −18.25 rpsH Ribosomal protein S8 Translation
    CheA −8.5 cheA Two-component sensor kinase Motility and chemotaxis
    YjlCb −8 yjlC Unknown Protein of unknown function
    AtpF −7.5 atpF ATP synthase ATP synthesis
    FliL −6.5 fliL Flagellar protein Motility and chemotaxis
    LiaH −6 liaH Phage shock-like protein Resistance against oxidative stress and cell wall antibiotics
    Spo0M −6 spo0M Unknown Sporulation
    FlgG −5.5 flgE Flagellar hook protein Motility and chemotaxis
    GgaB −5 ggaB Membrane protein Biosynthesis of teichoic acid
a

According to the SubtiWiki database (77).

b

Protein detected in both cytosolic and membrane fractions.

HHS Vulnerability Disclosure