FIG 2.

Effect of hrmK on accumulation of HmpD, PilA, and HPS. (A) Immunoblot analysis of HmpD, PilA, and RbcL in the wild-type and ΔhrmK mutant strains (as indicated) 0 and 24 h after hormogonium induction. RbcL is the large subunit of RuBisCO and serves as a protein loading control. (B) Fluorescent lectin staining of HPS in the wild-type and ΔhrmK mutant strains (as indicated). Depicted are merged images of fluorescence micrographs acquired using a 10× lens objective from cellular autofluorescence (red) and HPS (yellow) (strains as indicated) 0 and 24 h after hormogonium induction.