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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Biotechnol Bioeng. 2019 Dec 18;117(3):886–893. doi: 10.1002/bit.27234

Figure 2. Circuit construction using the Yeast Toolkit scheme:

Figure 2

Part plasmids contain unique upstream and downstream BsaI-generated overhangs to assemble into the appropriate position in “cassette” plasmids. Cassette plasmids are fully functional transcriptional units that are further assembled into multigene plasmids using BsmBI assembly and appropriate Assembly Connectors. This figure utilizes the color scheme and organization from Lee, et al 2015 to illustrate how the new optogenetic components integrate with an existing yeast toolkit.