Fig. 1.

Time course of JNK expression during epileptogenesis. A. Example western blot of JNK expression in rat CA1 hippocampus during the chronic epilepsy phase (6–9 weeks post-status epilepticus [post-SE]) compared to age-matched controls. When probed with a primary antibody that recognizes either all phosphorylated JNK isoforms (pJNK) or JNK isoforms regardless of phosphorylation state (total JNK), JNK expression is seen in two electrophoretic bands at 54 and 46 kDa. Shown are four different protein loading amounts in each condition, and a molecular weight standard in the leftmost lane. GAPDH expression was measured as a control for protein loading. B. Time course of pJNK expression during epileptogenesis shows that there is no significant change at times from 1 h to 1 week post-SE. In chronic epilepsy 6–9 weeks post-SE, there is significant elevation of pJNK expression in both 54 and 46 kDa bands compared to control. C. Total JNK expression during epileptogenesis shows no significant change at any time point post-SE. D. The phosphorylated fraction of JNK expression shows significant increases for both 54 and 46 kDa bands in chronic epilepsy and at some intermediate time points during epileptogenesis.