Fig. 2.

Segregation of JNK isoforms into 54 and 46 kDa bands. A. Western blots from a single sample probed with a pan-specific antibody against total JNK (all three isoforms, irrespective of phosphorylation state) and with an isoform-specific JNK1 antibody. Shown are three different protein loading amounts in each western, and two lanes with molecular weight standards. The JNK1 isoform migrated predominantly but not completely in the 46 kDa band. B. The JNK2 isoform migrated nearly equally in the 54 and 46 kDa bands. C. The JNK3 isoform was found almost but not entirely in the 54 kDa band. D. Quantification of the percentage of expression of each JNK isoform in the 54 and 46 kDa bands.