Figure 4.
miR-203a-3p/SOCS3 Axis Regulates RPE Differentiation
(A and B) Relative expression of SOCS3 mRNA (A) and protein (B) in ARPE-19 cells transfected with NC mimic, miR-203a-3p mimic, NC inhibitor, and miR-203a-3p inhibitor. (C) JAK/STAT enrichment analysis. (D) Expression of SOCS3 along with RPE differentiation. (E) Expression of SOCS3 in diethyl pyrocarbonate (DEPC)- and H2O2-treated RPE cells. (F) Expression of SOCS3 in DEPC- and TNF-α-treated RPE cells. (G) We used immunofluorescence staining to visualize RPE characteristic markers ZO-1 and β-catenin in ARPE-19 cells transfected with scramble siRNA, SOCS3-siRNA, and SOCS3-siRNA together with miR-203a-3p mimic. Scale bar, 20 μm. (H) Immunofluorescence staining was applied to compare expressions of ZO-1 and β-catenin in ARPE-19 cells transfected with scramble siRNA, LINC00167-siRNA, and LINC00167-siRNA together with AcFLAG-SOCS3. Scale bar, 20 μm. The data are presented at as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.