Figure 2.

circHUWE1 Structure and Expression Profiles

(A and B) The head-to-tail splicing of circHUWE1 was confirmed by Sanger sequencing (A), and the secondary structure was predicted (B). (C) PCR and agarose gel electrophoresis assay with divergent or convergent primers indicated the existence of circHUWE1, and circHUWE1 was shown to mainly localize to the cytoplasm. (D and E) Resistance to RNase R (D) and actinomycin D (E) was tested using qRT-PCR assay, and the results indicated that circHUWE1 was more stable than linear HUWE1. (F–I) Expression of circHUWE1 and linear HUWE1 in bovine tissues from fetal calf (F) or adult cattle (G), and myoblast-induced proliferation and differentiation (I) was measured, revealing wide expression with a significant decrease during myogenesis (H). *p < 0.05, **p < 0.01.