Fig. 3.

Design of chimeric CAZymes for synthetic fungal cellulosomes. A) For optimal dockerin placement, the dockerin and linker sequence was taken from the closest-related sequence in P. finnis and grafted in the same location on the on chimeric T. maritima CAZymes. The CMCase or Xylanase activity at 80 °C was comparable for both the native enzyme and the enzyme with the grafted dockerin domain for both a GH5 (Cel5A, panel B) and a GH10 (XynA, panel C). Significance was tested using the Mann-Whitney U test and determined not significant for each set of enzymes. Units (U) are defined as the amount of enzyme required to release 1 μmol of reducing sugar per minute.